How can I obtain an accurate and optimal melting curve analysis result in evaluating miRNA expression levels using the miRCURY LNA SYBR Green?

I performed a qPCR experiment using QIAGEN's miRCURY LNA RT kit and miRCURY LNA SYBR Green Kits to investigate the miRNA expression levels in some genes, but I could not obtain the desired result...

08 June 2022 1,683 0 View