Serap Demirel

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Questions related from Serap Demirel

Is anyone can help me about cutting pET32a expression vector?

I am going to clone a gene into pET32 a expression vector. After completing the cloning process, I verified my insertion by PCR an I see about 800 bp product . Then also, I wanted to verify with...

13 October 2020 7,073 2 View

Cutting pET32a expression vector

I am going to clone a gene into pET32 a expression vector. After completing the cloning process, I verified my insertion by PCR an I see about 800 bp product . Then also, I wanted to verify with...

01 January 1970 1,369 6 View

Double digestion PstI and EcoRI (pDUET-1)

I have problems double digest With my newly arrived primers, I amplified the gene I transferred into pGEM-T-Easy vector by PCR. At the same time, I amplified the corresponding gene from wheat...

01 January 1970 727 4 View