Serageldeen Sultan

7 Questions 16 Answers 0 Followers

Questions related from Serageldeen Sultan

How can I use the Cross Hi test for antibody evaluation?

Is there any standardized protocol to do Hi test for evaluating the antibodies against vaccine virus strain and mismatched of these antibodies by wild viruses

12 December 2015 6,414 3 View

Is the Tm calculation equation for degenerative primer the same as the ordinary one or there is a special equation for it?

As I showed in two papers they use the same primer but each one used quite different Tm (57C and 46C). Why? For your knowledge this is the primer sequence 5'TGG CGC ACY GTW TCY CAC CG3'.

10 October 2014 547 2 View

Is there a relation between NP antibodies and HA antibodies?

I did a survey for AIV in bird serum by ELISA (coated with NP antigen) and HI (H5 and H9) tests. However, there are samples that give positive results by HI but were negative by ELISA. Is there a...

11 November 2013 9,012 5 View

Non-specific PCR product?

I have used the primers VC-F cgg cgt ggc tgg ata cat tg and VC-R gtc aca ctt aaa tag tag cgt cc to amplify 389bp specific for V. cholera but every time a small band less than 100bp appears...

06 June 2013 8,791 15 View

What are the reasons that lead to the inability of stacking gel to become polymerized and still remain liquid?

I checked the pH of tris (6.8) and fresh preparation of APS solution. What is the reason?

02 February 2013 4,566 18 View

What is the exact Tm of this primer pair?

I need help to determine the Tm of this primer pair Primer F 5' ATCCCAAGCCTTCTACC 3' Primer R 5' GCCACAGCAGTGTTTTCT' 3' I tried several annealing temp. 47 , 50, 51 and 52C but no...

10 October 2012 789 6 View

Estimation of the cellular immunity

Hello, I am going to do an experiment about the estimation of the cellular immunity, specially that convey by T cells in immunosupressive viral infection. So, I am wondering Is there a simple...

04 April 2012 6,055 6 View