Senthilvelrajan Kaniyappan

4 Questions 5 Answers 0 Followers

Questions related from Senthilvelrajan Kaniyappan

Screening of Interacting partners! Is ImmunoAffinity Chromatography Suitable?

Hello, I would like to find the interacting partners of my protein of interest (POI) in tissue homogenates. The antibody specific to the protein of my interest is available. Since I would be using...

20 September 2016 2,970 4 View

Is it scientific if I keep 5 hippocampal slices from 5 different pubs/insert and consider them as N=5? and treat them the way I want?

I would like to treat the hippocampal slices with abeta oligomer. If I place 5 slices from 5 different pubs in a single insert, I guess I can consider them as n=5. Is that ok? Does anyone do this...

06 December 2013 3,123 3 View

Why do we have to do the a beta oligomer treatment to the hippocampal slices in a serum free medium?

Many articles mention that they treat the slices with a beta in serum free medium. why do they do this?

06 December 2013 6,580 1 View

Is it possible to have H2-DCFDA in the medium for 72 hours without washing?

I would like to see the increase in the ROS level in organotypic slice culture upon treatment with my compound. This compound is extremely precious, as it takes months and months produce in the...

15 November 2013 5,897 2 View