9 Questions 6 Answers 0 Followers
Questions related from Sena Nur
Dear researchers, greetings to all of you There is a situation that is not in my field of study but that I need to understand for an experiment. I isolated lysogenic phage, performed many...
29 March 2023 2,072 5 View
Hello everyone, After precipitating the proteins with ammonium sulfate, we performed SDS-PAGE. However, there was a problem with the gel running, we could not see any band. We did dialysis to...
14 February 2023 7,555 2 View
The lysogenic phage genome, which I isolated by the traditional phenol-chloroform method, appears small on 0.6% agarose gel electrophoresis. When I compare it with Marker, it is equivalent to 250...
17 January 2023 3,527 7 View
Hello, We will use Tricine SDS Page protocol to seperate our low kDa proteins. In this protocol, there are anode and cathode buffer but we can not understand how should we add these buffers ?...
30 March 2022 9,751 3 View
Hello, I want to discard sodium salt from drug (Nafcillin) ? We want to Nafcillin without salt, insoluble form in water ? So, is there any technic that we can apply ?
26 October 2021 5,984 0 View
25 October 2021 5,941 1 View
I will bring bacteria and receptor binding protein together, protein binds bacteria. Is there any differences between absorbances of just bacteria and after interaction of bacteria+receptor...
01 June 2021 9,301 3 View
I want to generate periplasmic protein of another bacteria in E. coli BL21 strain ? How can i do this ? I verified the cloning with pcr but protein production is not happening. Anyone have a...
30 May 2021 4,599 3 View
I am doing ELISA, where I am testing the binding of proteins to the cells. During ELISA, when i read results, i see OVRFLW (overflow) signal. So i can't understand the absorbances. Also, TMB...
04 May 2021 4,373 3 View
