Selami Demirci

5 Questions 8 Answers 0 Followers

Questions related from Selami Demirci

Why do 3T3-F442A cells detach from dish surfaces?

We are working on adipogenesis using various in vitro models. One of them is 3T3-F442A cells. In the literature, there are numerous protocols for differentiation of these cells. However, mostly...

02 February 2016 2,495 9 View

What is the correct time for PTEN inhibitor treatment in in vitro?

We have determined that our gene overexpression in dermal fibroblasts resulted in decreased cell proliferation and migration. Western blotting results showed that this effect is due to inhibition...

01 January 2015 2,187 3 View

What is the correct incubation time for experiments in gene overexpression studies?

We are currently conducting gene function studies using overexpression and knockdown approaches. We have observed that some transduced genes resulted in decreased proliferation of fibroblast...

11 November 2014 272 2 View

What are the best PI3K/Akt Signaling pathway antibodies for Western Blot?

I want to study PI3K/Akt Signaling pathway in human and mouse samples using Western Blot. Does anyone know antibodies for Akt signaling pathway intermediates which works great in Western Blot,...

10 October 2014 8,373 7 View

Is continuous puromycin treatment during the experiment necessary for gene therapy studies?

I have generated lentiviral mediated gene overexpression in fibroblast cells. I treated cells with puromycin (10 ug/ml) for 28 days and observe GFP (expressed as a reporter gene along with...

07 July 2014 1,000 5 View