Sehrish Javaid

3 Questions 4 Answers 0 Followers

Questions related from Sehrish Javaid

How clean is your CRISPR knockout?

Hi, I have been trying to get rid of two genes of my interest using CRISPR. siRNA knockdown suggested that those genes did not effect the viability of the cells so I should not be killing cells by...

20 November 2016 3,801 15 View

Is it normal for cells to grow in sheets in soft agar?

I am doing soft agar growth assays for the first time and my lab has not performed these assays extensively before either. I prepared my agar bases with 0.6% agar and suspended equal amount of...

01 January 2016 7,735 4 View

Has anyone experienced transfection failure for OKF cells?

I recently joined a lab and was handed a protocol and cells for transfection using fugene 6. I prepared the plasmid DNA and the GFP signal was good the first time. However ever since then, I have...

15 December 2015 462 3 View