Sehar Fazal

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Questions related from Sehar Fazal

Should we perform Heart puncture before collecting Bronchoalveolar Lavage from mice?

In my experiment, I needed to collect blood, BAL fluid, and lungs. I administered ketamine/xylazine anesthesia, followed by cervical dislocation, then performed cardiac puncture for blood...

29 July 2025 453 0 View

Impact of skipping overnight 4°C incubation (with RNA later) on RNA stability and downstream qPCR results?

Hello fellow researchers, I'm seeking advice on the potential consequences of deviating from the recommended protocol for RNA stabilization and extraction. Specifically, I collected mouse lungs,...

17 March 2025 9,705 5 View