I am trying to culture macrophages from bone marrow derived macrophages (BMDM) from mouse bone but BMDM are not dividing and differentiating?

I've been trying to cultivate BMDM into macrophages since a weak, and I'm using DMEM with 10% FBS and 5% pen strep. I changed the media three or four times and rinsed it with 1X PBS once. Although...

21 March 2022 5,116 3 View