Seda Homurlu

3 Questions 8 Answers 0 Followers

Questions related from Seda Homurlu

Is phenol-chloroform extracted DNA suitable for exome sequencing?

Hi. We isolated genomic DNA using the phenol-chloroform method and stored it at -80°C for approximately 15 years. Currently, we would like to use this DNA for exome sequencing analysis. Given...

27 April 2025 4,290 10 View

Is a specialized electrophoresis tank essential for Comet Assay, or can standard tanks suffice?

I’m planning to run the Comet Assay to assess DNA damage, but I’m debating whether it’s worth investing in a specialized electrophoresis tank or if a standard tank can handle it well enough. Has...

31 October 2024 5,329 4 View

Has anyone faced low transfection efficiency of SK-BR-3 and BT-474 cell with Lipofectamine 3000?

Hello. I've been trying to transfect plasmid DNA to my breast cancer cell lines (BT-474 and SK-BR-3) with Thermo Fisher's Lipofectamine 3000 reagent. According to the thermo fisher, those cells...

25 March 2024 9,522 5 View