Sebastián Vera Sandoval

2 Questions 1 Answers 0 Followers

Questions related from Sebastián Vera Sandoval

Which is the optimal optical density for toxical recombinant protein expression in E.coli BL21 (AI)?

Hi everyone. I am trying to express and purify a recombinant antimicrobial peptide using as a host cell E. coli BL21 (AI) (employing L-arabinose and IPTG). Because this peptide can be toxic to...

20 February 2020 4,997 4 View

Low transformation efficiency and expression of toxic genes in E.coli BL21-DE3 and BL21-AI

I cloned a toxic protein in E. coli BL21-DE3 and E. coli BL21AI, and the transformation efficiency was very extremely low (1-8 colonies). It is known that basal expression of the cloned genes can...

01 January 1970 5,058 3 View