Sebastián Tobar

2 Questions 2 Answers 0 Followers

Questions related from Sebastián Tobar

How to design a yeast integrative plasmid?

Hi everyone. I know that for an integrative plasmid you must add homology sequences to an specific locus where you want the integration, but i have doubts about regions that are usually prefered...

25 January 2022 8,841 2 View

Do restriction enzymes leave a 5 phosphate even in PCR products?

Hi, i am an undergraduate student and i'm trying to clone a gene fragment in a plasmid vector for the fist time for me. I have some questions, one of them is that i was reading that in some...

11 July 2021 9,808 3 View