Sebastian G Alvarado

3 Questions 9 Answers 0 Followers

Questions related from Sebastian G Alvarado

What protease cocktails do you use to disassociate tissues for cell sorting?

We are looking for diverse approaches to disassociating fin tissues from a fish. We are currently using TrypLE but we've got a feeling that spiking it with additional proteases may get the job...

10 March 2020 8,405 3 View

Does anyone have recommendations for a Tg-Mouse that ubiquitously expresses GFP/YFP/RFP/etc. in ALL neurons?

Ideally would like STRONG widespread expression in dendrites ideally in C57 background.

03 October 2017 2,741 2 View

Can someone offer advice on choosing a non-toxic brittle hydrogel that remains optically clear after polymerization?

I want to avoid using something like agar (avoid boiling) or polyacrylamide (toxic). I want to make deformations in the gel that I will be able to clearly see from all sides of a glass jar. Also,...

07 October 2015 8,588 9 View