Sean Cuninghame

6 Questions 3 Answers 0 Followers

Questions related from Sean Cuninghame

Can anyone help with Fluorescent Glucose (NBDG) analogs for glucose uptake analysis in cell culture?

I am interested in determining the amount of glucose that is taken up by a couple of cancer cell lines. I have tried a couple of commercial kits to no avail, so am attempting to use NBDG and...

03 March 2014 3,509 4 View

Does anyone have experience using Native PAGE for PKM2 detection?

I would like to detect PKM2 oligomerization state (dimer vs. tetramer) in a variety of cancer cells. Most literature I have noticed use Size Exclusion Chromatography to answer this but Native Gel...

02 February 2014 3,114 5 View

Measuring membrane transport activity - how long following trypsinization?

I want to determine glucose uptake in epithelial cells. I trypsinize the cells from the a stock flask and seed out a desired amount of cells in 96 well plates to perform the assay in. I am unsure...

02 February 2014 7,125 3 View

Identity of high molecular weight band in HIF1alpha in Western Blot?

I have been doing Western blots for HIF1alpha in cancer cell lines. The predicted band size of HIF1alpha is ~116 kDa. I get a band at this size but also typically get an additional band at ~200...

08 August 2013 4,930 5 View

Membrane choice for Western blotting of HIF-1

I am trying to perform Western blots for HIF-1alpha. I have so far had one successful attempt using a nitrocellulose membrane. When I attempt to perform the same lysis protocol, gel running...

05 May 2013 3,619 8 View

Experimental design for Western Blot semi-quantification?

I have six treatment groups (different cell types) with four biological replicates per group that I would like to compare protein levels in. I have come up with several experimental designs, each...

01 January 2013 7,310 11 View