Sean Pollen

3 Questions 2 Answers 0 Followers

Questions related from Sean Pollen

How can I normalise two-photon confocal-microscopy fluorescence measurements?

I would like to be able to compare fluorescence measurements taken at different times in different samples directly with one another. From previous experience I know that microscope settings...

19 June 2017 1,761 2 View

How can I isolate rat proximal tubule cells for immediate respirometry?

I would like to take rat kidney tissue and isolate from that the proximal tubule cells for immediate use in a Clark-type oxygen electrode to study mitochondrial respiration. I do not want to study...

15 June 2017 1,937 2 View

How can I correct for photobleaching in time lapse confocal microscopy imaging?

I'm consistently having problems with this and haven't figured out how to solve it. Are there good approaches to avoiding bleaching in the first instance? Is creation of a photobleach curve...

02 December 2016 8,843 3 View