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We have been using 45°C on our Columbus Instruments Hot/Cold Plate Analgesiometer with 3-6 month old Sprague-Dawley rats. Previously, this gave us a reliable baseline latency of 100-200 seconds...
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I have an old (at least 10 years) bottle of progesterone from Calbiochem and would like to confirm the biological potency. Does anyone know of a reasonably simple biochemical or biological assay...
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