Scott Godwin

4 Questions 5 Answers 0 Followers

Questions related from Scott Godwin

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

30 July 2020 3,980 2 View

How do you measure OD600 of Pichia pastoris when it flocculates?

I've been struggling to get consistent results from my Pichia transformations. I've tried both heat shock and electroporation protocols. I suspect that part of my problem is that the cultures tend...

30 April 2018 5,239 3 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

21 December 2017 9,991 5 View

Methanol concentration for induction of Pichia pink strains?

I'm trying to use the Pichia pink strains supplied by Invitrogen to express recombinant proteins. So far I've identified a few clones that express my proteins intracellularly, but the protein...

21 December 2017 6,577 1 View