Scott Godwin

3 Questions 4 Answers 0 Followers

Questions related from Scott Godwin

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

30 July 2020 3,912 2 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

21 December 2017 9,937 5 View

Methanol concentration for induction of Pichia pink strains?

I'm trying to use the Pichia pink strains supplied by Invitrogen to express recombinant proteins. So far I've identified a few clones that express my proteins intracellularly, but the protein...

21 December 2017 6,546 1 View