How can I improve the efficiency of the PCR amplification of my vectors (pET_His_1a, pET_Trx_1b, pET_DsbC_1b)?

I have tried to run PCR for the vectors under teh following conditions: Step 1: 95°C for 2 min Step 2: 95°C for 1 min Step 3: 55°C for 1 min Step 4: 68°C for 2 min Step 5: Cycle to Step 2 25 more...

01 January 1970 3,443 4 View