Quantification of FRET for two fluorophores with similar lifetime and overlapping emission spectra?

Donor is rsFastLime (496 nm excitation max/518 nm emission max) and Acceptor is EYFP (514 nm excitation max/525 nm emission max). To quantify FRET, if we want to measure the rsFastLime...

13 January 2023 9,721 6 View