Sayan Kundu

3 Questions 3 Answers 0 Followers

Questions related from Sayan Kundu

For Methanol/ chloroform protein precipitation what is the optimal ratio??

I am using methanol/ choloform mixture for protein extraction from other interferring biomolecules, e.g: lipid, other peptides etc. Now I use 200 ul of protein sample with 3: 0.75: 2 volumes of...

18 May 2021 7,536 0 View

How Can I get rid of multiple bands in my SDS PaGE? My western blot is showing the protein band sharply, but that also conclude low yield??

I am trying to purify the galactosaminyl transferrase, LgtD. I used BL21 E coli strain, lysis buffer (EDTA+NaCl+Tris Hcl) and washing buffer (imidazole conc 5 mM) and Elution buffer (20mM...

13 May 2021 9,569 2 View

How can I quantify the carboxylic acid group on carbon nano surface

We actually want to conjugate a dendrimer on Carbon nano particle that's why we require no of carboxylic acid group on carbon nano surface

01 January 1970 9,358 2 View