Savio Cardoza

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Questions related from Savio Cardoza

While running a sample analysis in Waters HPLC binary system, I ofter get a completely straight baseline at minus absorbance value.Why?

I am running a gradient of ACN and Water in 4.6 x 250 mm C18 RP column. Column temp is 25 degrees and flow rate is 2 ml per min. I go from 50:50 (Water:ACN) to 1:99 (Water:ACN) over 10 min. I...

17 January 2022 6,922 4 View

While doing HPLC in gradient elution at what time should we inject our analyte?

Im doing HPLC analysis for organic compound. I am not able to understand at what time should I inject the sample. The reason being that im starting with 5% A :95% B. If I inject a sample at two...

19 March 2021 7,758 5 View

I am getting peaks in the aliphatic region always in NMR after doing column. It has grease peaks. How can i removie it?

I am getting peaks in the aliphatic region always in NMR after doing column. It has grease peaks. I do not get a clean NMR and washing with pentane does not effect much,

28 January 2018 5,334 6 View

How can i purify N-oxides on column chromatography?

What solvent system to use to get purified N-oxide compounds in column chromatography?

03 February 2017 8,140 3 View