Saverio Charalambous

4 Questions 5 Answers 0 Followers

Questions related from Saverio Charalambous

Why does my PCR amplification produces non-specific bands in a consistent manner?

Hello, I am running a PCR amplification using NEB Q5 high fidelity polymerase kit on 1.4-1.6 kb gBlock fragments with the following reaction conditions: > 30s at 98°C > 10s at 98°C -...

29 July 2019 1,894 4 View

Weird Black Line on Western blot ?

I have some gels that have a consistent black line at unexpected protein size consistent throughout gel? What could be the reason? Thank you

26 July 2019 7,272 3 View

Weird Black Line on Western blot ?

I have some gels that have a consistent black line at unexpected protein size consistent throughout gel? What could be the reason? Thank you

07 July 2019 6,496 3 View

Why does my PCR amplification produces non-specific bands in a consistent manner?

Hello, I am running a PCR amplification using NEB Q5 high fidelity polymerase kit on 1.4-1.6 kb gBlock fragments with the following reaction conditions: > 30s at 98°C > 10s at 98°C -...

07 July 2019 9,414 4 View