Saulo Cruz

3 Questions 1 Answers 0 Followers

Questions related from Saulo Cruz

What would be the optimal immunofluorescence protocol for detecting TGF-beta in mouse cerebral cortex tissue?

I'm facing challenges with performing immunofluorescence for TGF-beta along with GFAP in my 4% PFA-fixed brain samples (frozen sections). While GFAP is yielding satisfactory results, TGF-beta...

03 October 2023 9,645 0 View

What is the best antigen retrieval for the immunofluorescence protocol for iNOS in brain tissue fixed with 4% PFA?

My iNOS stainings are not working. I am using the Santa Cruz Biotech anti NOS2 antibody (sc-7271), but I have only been getting non-specific stainings. Has anyone ever used this antibody on...

06 December 2021 5,340 3 View

Is it possible to quantify proteins by western blotting in brain fixed with 4% paraformaldehyde?

My group and I have some samples of frozen mouse brains that are fixed in paraformaldehyde. However, tissue quality does not allow us to perform the immunofluorescence protocol. So, so that we...

03 December 2021 5,940 2 View