Saugat Pokhrel

2 Questions 6 Answers 0 Followers

Questions related from Saugat Pokhrel

What could be the possible critical reasons for failure of transient gene expression in onion epidermal peels using PDS 1000He device?

Hi, I have worked with onion epidermal cell transformation for a long time now. Did some successful transformations with high efficiency towards the end of last year and beginning of this year...

10 October 2016 7,044 8 View

Can anyone suggest a good reliable method, percent of gel to be used or other alternatives?

I want to detect a digested product of 62 bp in an agarose gel. I am doing a Site Directed Mutagenesis and want to screen my colonies. The mutation introduced a restriction site XhoI. Another XhoI...

08 August 2016 8,846 2 View