Sathi Mallick

3 Questions 3 Answers 0 Followers

Questions related from Sathi Mallick

How can a exopolysaccharide (EPS) band be eluted from silver stained,12% PAGE/8M urea gel?

I have run a 12% SDS-PAGE/8M Urea gel for my bacterial EPS sample and have got bands for the polysaccharide after silver staining it. I need to elute the desired band for further analysis of the...

06 June 2016 6,355 4 View

Is it possible that on infection, the macropage iNOS and TNFalpha mRNA expression to be high whereas its NO and TNF production to be low in vitro?

I have infected the mactrophage RAW 264.7 with E. coli and studied its NO and TNF alpha production in vitro several times which shows each of their low production respectively. However, when the...

12 December 2015 7,489 2 View

Can Arabinose be used as a separate component in DMEM for RAW 264.7 cell lines during a coculture experiments?

I am doing a coculture experiment where I need to infect the RAW cells with E. coli. The E. coli cells used however are induced with arabinose. So do I need to add arabinose to the DMEM media too...

07 July 2015 9,658 3 View