Sathi Mallick

4 Questions 3 Answers 0 Followers

Questions related from Sathi Mallick

What is the reason of getting non specific spots in Fluorospot/ ELISpot assay?

While performing a Fluorospot assay, generally there should not be a situation where non-specific spots are observed. However, after performing the experiment multiple times by multiple people in...

03 June 2022 9,451 0 View

How can I homogenize the mice tissue to isolate & count the infected bacteria from within it, i.e without lysis of these bacteria during the process?

I have fed the 2-3 weeks old swiss albino mice with E. coli (by gavage) and am trying to get a count of these bacteria that has caused infection in the colon region. After oral gavage the mice...

21 December 2016 2,434 4 View

How can a exopolysaccharide (EPS) band be eluted from silver stained,12% PAGE/8M urea gel?

I have run a 12% SDS-PAGE/8M Urea gel for my bacterial EPS sample and have got bands for the polysaccharide after silver staining it. I need to elute the desired band for further analysis of the...

14 June 2016 8,794 3 View

Can Arabinose be used as a separate component in DMEM for RAW 264.7 cell lines during a coculture experiments?

I am doing a coculture experiment where I need to infect the RAW cells with E. coli. The E. coli cells used however are induced with arabinose. So do I need to add arabinose to the DMEM media too...

09 July 2015 5,506 3 View