Saswat KUMAR Mohanty

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Questions related from Saswat KUMAR Mohanty

What is the excitation light for YFP protein?

I have C.elegans strain with YFP tagged to my protein of interest. So to visualize the YFP in fluorescent microscope which excitation light should I use?

03 March 2019 5,783 2 View

Can any one help to prepare tissue sample for western blot? And how much concentration of protein should I load?

I used to prepare 10% tissue homogenate by weighing the tissue in RIPA with protease and phosphatase inhibitor. After homogenization I used to centrifuge it for 20min at 13000rpm in 4 degree....

02 February 2019 9,586 10 View