Sasmita Samal

5 Questions 7 Answers 0 Followers

Questions related from Sasmita Samal

What is the concentration of PKH67 dye to be taken for tagging exosomes? How to get rid of excess and unbound dye?

I am trying to tag isolated exosomes with PKH67 dye, but every time after doing one round of ultracentrifugation (2,00,000Xg, 90 min) I see some pellets in the control sample with PBS. I am...

18 December 2019 1,098 0 View

Has anyone seen any pellet after isolating exosomes by ultracentrifugation?

I have isolated exosomes from conditioned cell culture supernatants, but have not witnessed any pellet after two steps of ultracentrifugation. Is it normal or should I must see some pellets? Any...

22 April 2019 5,200 5 View

Is there any method available to increase the yield of exosomes from cell culture media?

I am working on MC3T3-E1 cell line and isolating exosomes from cell culture media by using standard ultracentrifuagtion method. I have characterized with TEM and checked the size and charge with...

06 April 2019 9,631 5 View

Why the upcoming science is more focused towards using exosomes as drug delivery vehicles, while liposomes are already serving as good models?

Nowadays extensive research is going on modifying exosomes and using them as potential drug delivery vehicles. But if I am not wrong, lipososmes and some other natural nano-particles are some of...

14 February 2019 4,874 6 View

After keeping cells with serum free media for 16 hrs can we use these again for some experiments?

I am working on exosome isolation from conditioned media. I am using MC3T3-E1 cell line as the source. Before going for isolation, I used to keep the cells with serum free media for 16 hrs. Any...

08 February 2019 4,193 5 View