Sasmita Samal

4 Questions 6 Answers 0 Followers

Questions related from Sasmita Samal

What is the concentration of PKH67 dye to be taken for tagging exosomes? How to get rid of excess and unbound dye?

I am trying to tag isolated exosomes with PKH67 dye, but every time after doing one round of ultracentrifugation (2,00,000Xg, 90 min) I see some pellets in the control sample with PBS. I am...

18 December 2019 1,073 0 View

Has anyone seen any pellet after isolating exosomes by ultracentrifugation?

I have isolated exosomes from conditioned cell culture supernatants, but have not witnessed any pellet after two steps of ultracentrifugation. Is it normal or should I must see some pellets? Any...

22 April 2019 5,168 5 View

Is there any method available to increase the yield of exosomes from cell culture media?

I am working on MC3T3-E1 cell line and isolating exosomes from cell culture media by using standard ultracentrifuagtion method. I have characterized with TEM and checked the size and charge with...

06 April 2019 9,594 5 View

After keeping cells with serum free media for 16 hrs can we use these again for some experiments?

I am working on exosome isolation from conditioned media. I am using MC3T3-E1 cell line as the source. Before going for isolation, I used to keep the cells with serum free media for 16 hrs. Any...

08 February 2019 4,162 5 View