Sasmita Dash

2 Questions 2 Answers 0 Followers

Questions related from Sasmita Dash

Problem with beta galactosidase staining assay: X-gal crystals in staining solution?

Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...

26 March 2024 8,676 3 View

Cell debris or contamination in fibroblast culture?

I am culturing human primary fibroblasts obtained from skin punch biopsy, in DMEM +10%FBS+ 1% Ciproflaxin. I see these weird structures floating on the media, however no movement or color change...

27 December 2022 7,002 3 View