I am using the PCR machine to make c-DNA from m-RNA and used different timings , will this affect my results ?

As an example, I suppose to keep the cycle 1 in 22 C for 5 mins while I kept it for 5 seconds.....42 C for 30 minutes while i only kept it for 3 seconds. I guess when I was entering the numbers...

02 February 2020 1,151 3 View