Saroj Chandra Lohani

5 Questions 11 Answers 0 Followers

Questions related from Saroj Chandra Lohani

Anybody like to share complete protocol for gene knock out of Nicotiana tobacum using CRISPR Cas9?

I am using CRISPR Cas9 system to knock out my gene of interest in Nicotiana tobacum. For this I need a complete protocol explaining each and every step with reaction mixture. Anybody sharing a...

15 January 2017 4,108 5 View

What are the ATCC cultures (gram positive and gram negative) should be used in testing antimicrobial activity of herbal plant Extract?

I have 10 MDR isolates (five each of gram positive and gram negative bacteria) including metallo beta lactamase producer isolated from clinical samples and I want to test antimicrobial effect of...

22 July 2015 2,843 2 View

How to calculate chi square test for more than 2 by 2 consistency table in SPSS?

If we want to calculate chi square test for 3 by 2 or 4 by 2 or more than 2 by 2 consistency table, what is the procedure in SPSS? If some cell has expected value less than 5 then what will be the...

04 December 2013 1,610 9 View

What is the mechanism beyond Aztreonam (Monobactam) resistance in Metallo beta lactamase (MBL) producing Pseudomonas aeruginosa?

Literature suggests MBL producers are not able to hydrolyze Aztreonam (Monobactam) but during my thesis work I found most of the MBL producer Pseudomonas aeruginosa isolates are resistant to...

25 November 2013 7,041 4 View

What are the Zone Diameter breakpoints of Colistin (25 microgram) for Pseudomonas aeruginosa?

According to CLSI guideline Zone Diameter breakpoints of Colistin (10 microgram) for Pseudomonas aeruginosa are ≥ 11 mm for sensitive and ≤ 10 mm for resistance but if we use Colistin (25...

01 August 2013 8,341 3 View