Sarath Chandra Dantu

2 Questions 17 Answers 0 Followers

Questions related from Sarath Chandra Dantu

Electrostatic interactions between protein, dna, water interface?

I am working on a Protein-DNA complex and I have a question regarding the electrostatic interaction energies calculated at the interface of protein, DNA and water. I am using amber99sb+parambsc0 ...

27 December 2017 4,831 2 View

How to prevent DNA binding protein precipitation while concentating?

I have DNA binding protein and I am getting a conc, of 50um from 50ml BL21 DE3 culture (I will be scaling this up to 1mM). The protein is Hexa-His tagged at C-terminus and to elute the protein...

24 August 2017 9,338 3 View