Saranpal Chhabra

5 Questions 19 Answers 0 Followers

Questions related from Saranpal Chhabra

Cracks on retinal eye of P0 mice post H&E staining

I recently performed H&E staining on my paraffin embedded P0 mice tissues to visualize the phenotype of the eye in one of my WT mice. I managed to slice the tissues and stained them with...

22 May 2017 2,951 3 View

Can someone please explain why there are 2-3 bands of my protein after purification?

I realized that this often happens only when I perform purification. My protein is fused to a 60kDa tag and my protein it self is ~20kDa. So taken together, the fusion protein should result in...

01 August 2014 7,154 22 View

What do you think about my total RNA?

I have recently extracted total RNA from the bark of Rubber tree. However, I am not sure if these RNA's are contaminated with gDNA? I see some smears on the RNA. Could the smears represent gDNA?

09 July 2014 8,368 6 View

What is the cheapest and most convenient method of liberating/extracting gDNA from Pichia pastoris?

I have performed transformation of my gene into Pichia to express my desired recombinant protein. Now that I have successfully transformed, I would like to know if my gene has been integrated into...

02 January 2014 4,197 3 View

How do I quantify the DNA accurately on agarose gel?

Suppose that I am using 1kbp DNA marker

27 July 2013 7,694 23 View