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I've been using the Cytoskeleton G/F- actin in vivo kit (https://www.cytoskeleton.com/bk037) on my lysates, and am a stand-still with data analysis. I'm torn between whole protein normalization...
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I'm new to Western densitometric analysis, but I have watched various videos and read articles on how to conduct the analysis. My biggest problem is obtaining the peak percentage output on ImageJ....
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