Sarah Roelle

2 Questions 2 Answers 0 Followers

Questions related from Sarah Roelle

What loading control do you use for Western blot of secreted proteins in cancer cell lines?

I am doing a Western blot of an isoform of fibronectin (EDB) that is present in cancer. Normally, I would use beta-actin as a loading control. However, fibronectin is secreted into the matrix,...

10 October 2016 9,350 4 View

How can I prep lysates for Western blot that include my cells and the surrounding matrigel for cells grown in 3D culture?

I am interested in performing a western blot on cells that I have grown in Matrigel. I have read different protocols on recovering the cells from the matrix, including use of ice-cold PBS, Corning...

03 March 2016 6,012 1 View