Sarah Richards

5 Questions 4 Answers 0 Followers

Questions related from Sarah Richards

Why does enzyme not show activity when purified with BugBuster?

I have expressed my recombinant protein in E.Coli with a N-terminal his-tag. Cell lysis was done with BugBuster, purified successfully with IMAC (Co-resin) and the immidazole removed through a...

10 September 2019 1,453 3 View

Why does enzyme not show activity when purified with BugBuster?

I have expressed my recombinant protein in E.Coli with a N-terminal his-tag. Cell lysis was done with BugBuster, purified successfully with IMAC (Co-resin) and the immidazole removed through a...

09 September 2019 3,918 3 View

What is the best statistical test I can use to measure significance of the difference in Vmax and Km ?

I'm measuring the differences in Vmax and Km between a Wild-type protein and a mutant. Upon acquiring the Vmax and Km from triplicate enzyme assays for each protein (WT and mutant), what is the...

12 August 2019 811 4 View

Does Sonication affect protein purification?

I am attempting to purify a protein with his-tag. Current lysis method includes his-lysis buffer (pH8) with 1% triton and 2minute sonication (pulse 10seconds, rest 40 seconds) after incubation...

07 July 2019 2,975 10 View

Is H2O2 agar plates or culture better for yeast spot assay?

Doing a yeast spot assay on viability of yeast cells upon exposure to H2O2. Was wondering if it was best to grow cells in liquid media with H2O2 before plating on regular agar plates, or to grow...

06 June 2019 4,843 3 View