Sarah Franco

2 Questions 3 Answers 0 Followers

Questions related from Sarah Franco

Any suggestions for PBMC/monocyte culture conditions (I get cells to attach well after isolation but then they die the next day)?

I have tried isolating PBMCs (looking to culture monocytes and differentiate into macrophages) from human blood using Ficoll gradient. I get a good amount of cells that attach. After 2-3 hours I...

07 July 2019 858 6 View

Does anyone know the sequence for the multiple cloning site in Lonza's pmaxGFP or at least the order of restriction sites upstream of GFP?

Lonza provides a plasmid map of pmaxGFP showing the KpnI, NheI, Eco47III, and AgeI restriction sites upstream of max GFP and BglII, XhoI, and SacI downstream. Does anyone know if there are...

02 February 2015 8,636 12 View