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I have tried isolating PBMCs (looking to culture monocytes and differentiate into macrophages) from human blood using Ficoll gradient. I get a good amount of cells that attach. After 2-3 hours I...
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Lonza provides a plasmid map of pmaxGFP showing the KpnI, NheI, Eco47III, and AgeI restriction sites upstream of max GFP and BglII, XhoI, and SacI downstream. Does anyone know if there are...
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