Sarah Elizabeth Eysoldt

6 Questions 5 Answers 0 Followers

Questions related from Sarah Elizabeth Eysoldt

When conducting western blot experiments, do I have to load all experimental conditions on one gel, or is normalizing to total protein sufficient?

I am running 38 mice samples via western blot for 2 targets. I have already finished data analysis and have found significant differences between treatment groups. If I normalized all proteins of...

19 August 2019 5,871 3 View

When conducting western blot experiments, do I have to load all experimental conditions on one gel, or is normalizing to total protein sufficient?

I am running 38 mice samples via western blot for 2 targets. I have already finished data analysis and have found significant differences between treatment groups. If I normalized all proteins of...

08 August 2019 8,931 3 View

What is the best way to normalize phosphorylated protein expression?

I'm trying to analyze how the ratio of AMPKp: AMPK changes among my treatment groups. What is the best way to do this? Currently, all I've done is divide AMPKp by AMPK, and then calculate...

31 July 2019 5,214 3 View

Troubleshooting SYBR Green RT- qPCR?

I am trying to optimize my primers for qPCR using SYBR green chemistry. To do this, I pooled 10 of my mouse samples (control and experimental groups), and performed serial dilutions of 1:4 for a...

09 July 2019 5,208 5 View

Troubleshooting SYBR Green RT- qPCR?

I am trying to optimize my primers for qPCR using SYBR green chemistry. To do this, I pooled 10 of my mouse samples (control and experimental groups), and performed serial dilutions of 1:4 for a...

07 July 2019 8,971 6 View

What is the best way to normalize phosphorylated protein expression?

I'm trying to analyze how the ratio of AMPKp: AMPK changes among my treatment groups. What is the best way to do this? Currently, all I've done is divide AMPKp by AMPK, and then calculate...

07 July 2019 1,957 4 View