Sarah Boyle

5 Questions 12 Answers 0 Followers

Questions related from Sarah Boyle

What is a good approach to identify post-translational regulation by a protein in breast cancer?

There seems to be some crosstalk between two signalling pathways I am looking at in breast cancer primary cells. The knockout of one receptor inhibits the activation of the other, however, the...

03 March 2015 2,459 4 View

What are the best markers to analyze proportions of eosinophils by flow cytometry?

I'm working on primary mouse tissues and want to look at the proportion of infiltrating eosinophils in tumours using flow cytometry. Apart from CD45+, MHCII-, Gr1-, CD11b+, CD11c-, does anyone...

11 November 2014 8,697 9 View

Can anyone provide a protocol for identification of NKT cells in tumour tissues by flow cytometry?

I am trying to identify infiltrating NKT cells in mouse mammary tumours. I am not sure whether I still need to incubate cells with α-Galactosylceramide before detection with an anti-Mouse alpha...

10 October 2014 3,617 7 View

Can anybody recommend the best methods to study apoptosis?

I am trying to study apoptosis in primary mouse mammary cells. So far I have looked at 7AAD/Sytox and FLICA staining by flow cytometry, and upregulation of anti/pro-apoptotic proteins via Western...

09 September 2014 3,080 16 View

Is it necessary to perfuse mice when studying infiltrating immune cells in tumours?

I am interested in dissociating small, solid mammary tumours from MMTV-PyMT mice (which aren't necrotic or angiogenic) and using flow cytometry to assess levels of innate and active immune cells...

07 July 2014 7,536 8 View