Sarah Roelle

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Questions related from Sarah Roelle

What loading control do you use for Western blot of secreted proteins in cancer cell lines?

I am doing a Western blot of an isoform of fibronectin (EDB) that is present in cancer. Normally, I would use beta-actin as a loading control. However, fibronectin is secreted into the matrix,...

25 October 2016 7,921 3 View

How can I prep lysates for Western blot that include my cells and the surrounding matrigel for cells grown in 3D culture?

I am interested in performing a western blot on cells that I have grown in Matrigel. I have read different protocols on recovering the cells from the matrix, including use of ice-cold PBS, Corning...

22 March 2016 7,450 1 View