Sarah J McFetridge

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Questions related from Sarah J McFetridge

Why do I get different PCR melt curves for same primer in different sample sets?

I have run RT-PCR on 3 sets of HEK 293 samples and obtained divergent melt curves for one gene (Bovine RPLPO), while others show no issue. I carried out the RNA extraction and cDNA synthesis for...

07 February 2019 3,588 3 View

Why does LPS not increase monocyte/macrophage MMP-9 secretion?

I have stimulated primary human monocytes and monocyte-derived macrophages with 100 ng/ml E.coli-derived LPS for 24 hrs (monocytes) and 24/48/72 hrs (macrophages). Total MMP-9 ELISA on cell-free...

05 October 2018 102 5 View