Problems cloning HU (histone-like DNA binding protein) from a gram negative bacteria. Anyone else encounter problems cloning HU?

I am trying to clone HU, using pET-100/D-Topo vector and transforming them into TOP10 Electrocomp E.coli cells. After transformation I see a weird "lawn" (no colonies) on my selective plates....

31 July 2018 9,588 3 View