Sarah Dhaiban

6 Questions 10 Answers 0 Followers

Questions related from Sarah Dhaiban

Hi, what is the best marker for detecting presence of HCT-116 cancer cells?

I want to be able to detect the presence of HCT-116 micrometstasis in mice by staining the tissue with HCT-116 specific marker.

15 June 2020 2,718 3 View

Why the scramble siRNA (that is used in negative control) gives result different from the result of untransfected cells?

why the production of a protein by cells transfected with siRNA (in-vitro) is less than the production of that protein by untransfected cells? Thanks in advance

13 May 2017 8,236 2 View

Hi, why the sequencing peaks for the same nucleotide base (ex: for Adenine (green) in the attached picture) differ in their heights ?

Aren't they supposed to be the same height for the same nucleotide base?

21 October 2016 5,914 4 View

Hi, how can the monoclonal antibody that binds to antigen/receptor present in both normal and malignant cells, targets the malignant only?

As I was preparing the attached paper, it's mentioned that CD20 receptor is expressed in both normal and lymphoid cancer cells (page # 3, highlighted), yet, they designed monoclonal antibodies...

08 May 2016 5,724 7 View

Why is it that the OD for Rosetta cells doesn't increase ?

I inoculated Rosetta cells in 3 mL of LB with 3 uL of chloramphenicol for 16 hours and they showed growth (turbidity), then in the same day, I put 250 uL of them in a flask with 20 mL and 20 uL...

24 March 2015 446 10 View

What does it mean if the size of the plasmid that I extracted was smaller than the actual size ?

actual size of pAMP plasmid is 4.5 kbp while the one that I extracted was 2.7 kbp

01 November 2014 4,459 4 View