Sarah Dean

3 Questions 4 Answers 0 Followers

Questions related from Sarah Dean

Qubit vs Quantus, which is better and why?

Current application is library quantitation for NGS.

06 April 2023 274 1 View

Can qPCR products be sequenced in Ampliseq workflow?

We frequently have mixed results for a qPCR assay. We'd like to determine if this is non-specific amplification, or target amplification when the target is low-frequency. DNA tape-station shows...

03 February 2023 6,755 5 View

RQ-PCR, what are these mysterious false positives?

Our lab has an assay to detect fusion transcripts that very frequently results in "false positives." Often our duplicates show mixed results (one positive and one negative), always at a high C(t),...

10 January 2023 2,580 3 View