Using a ZymoPURE II Plasmid kit, what could cause clarified lysate to turn pink and cloudy after binding buffer addition, and result in low yield?

Hi all, I used a ZymoPURE II Plasmid Maxiprep kit to isolate plasmid DNA and ended up with very low yield and poor quality. Specifically, less than 10ng/uL in 500uL from 150mL of culture, and...

16 May 2024 6,753 2 View