Sarabjeet kour Sudan

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Questions related from Sarabjeet kour Sudan

Can anyone suggest me that why protein is not active after purification?

I am purifying a protein which is showing activity in sonicated supernatant but when i am purifying it using ion exchange, eluted fraction is losing the activity.I did dialysis also even then the...

01 December 2016 6,225 4 View

Why is the DNSA method showing no activity of my isolate although it shows activity on a starch azure plate?

I have an isolate that shows amylase activity on a starch azure plate incubated at 70°C but when I grew this isolate in broth, centrifuged it, and took its supernatant for performing assays, there...

24 February 2014 6,454 4 View