Why are my stem cells dying after lentiviral transduction(pLVX vector)? Too much RFP?

Hey folks, I have a problem transducing my human bone marrow derived mesenchymal stem cells. I transduce them with MOI of 4 and the use of 10 µg/ml polybrene. I have a pLVX vector with E2-Crimson...

31 July 2014 152 10 View