Sara Milani

3 Questions 4 Answers 0 Followers

Questions related from Sara Milani

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

23 July 2024 5,952 6 View

Endogenously tagging protein with His tag??

Hi everyone, There are challenges with using antibodies to capture my protein of interest so I will be using CRISPR to endogenously tag my RNA binding protein with a fluorophore followed by a His...

29 March 2023 8,637 1 View

Bacterial colonies growing on agar plate but not LB broth?

I transformed 5-alpha E.coli and plated onto freshly made agar plates with ampicillin (which has recently been made by another member of the lab). Less than 24 hours later I checked and my plate...

28 March 2023 4,424 6 View