Sara Kabir

3 Questions 2 Answers 0 Followers

Questions related from Sara Kabir

Can anyone tell me why my negative control GST is showing along with my experimental?

I am trying to do GST pull down with my experimental proteins and GST as my negative control. however, my negative control (GST) is interacting very strongly to my target protein and even lowering...

26 April 2023 8,226 0 View

GST pull down showing a band at the size of GST?

I am running a gst pull down assay on my GST (negative control) and p53 with His-Rev7. After the Western blotting i noticed my antiGST membrane is showing bands in the lanes of p53 a GST sized...

27 January 2023 2,596 3 View

KO cells concentration very low post buffer change?

I am working on Knock out cells and after protein purification their concentration seems to be good (ranging from ~0.185-0.191mg/ml) however, once I do the buffer change and do the Nanodrop the...

19 October 2022 9,937 0 View