Sara Amari

3 Questions 2 Answers 0 Followers

Questions related from Sara Amari

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

20 July 2024 5,216 2 View

Sanger sequencing problems?

Hello, I have problems with PCR fragment sequencing (Sanger sequencing on SeqStudio). We perform sequencing reaction with BigDye™ Terminator v3.1 with subsequent purification with BigDye™...

30 April 2024 4,581 3 View

Which is the better procedure to revive pneumococcal strains stored at -80C?

after identifying pneumococcus bacteria, i store it using 30% glycerol at -80 C. One month later i tried to reisolate it. All i have is a sterile blood plate with no growth.

11 March 2020 1,730 2 View